ABSTRACT
BACKGROUND@#How to locate pulmonary ground-glass nodules in thoracoscopic surgery is an important clinical topic in minimally invasive thoracic surgery. There is no unified localization method at present. This study intends to investigate the accuracy and security of body surface theodolitic puncture localization method in video-assisted thoracoscopic surgery for pulmonary ground-glass nodules.@*METHODS@#The clinical data of 41 patients from August 2018 to December 2019 were analyzed retrospectively, including 28 males and 13 females. After anesthesia, the patient was located by body surface theodolitic puncture, and then partial lobectomy was performed under video-assisted thoracoscopy. The distance from the nodule to the marked suture and the distance from the nodule to the incisal margin were measured, and the accuracy of localization, the rate of complication and the success rate of surgical resection were calculated.@*RESULTS@#A total of 51 nodules in 41 patients were located by body surface theodolitic puncture localization method. The accuracy rate was 96.1%, and the average location time was 8.3 min. Puncture bleeding occurred in 5 cases (12.2%), all of which were successfully stopped by video-assisted thoracoscopy, and there were no other complications. All patients underwent thoracoscopic partial lobectomy, including 33 cases of anatomical segmentectomy and 8 cases of wedge lobectomy. All the patients in operation process smoothly. The distance between nodule and incisal margin was measured, and all specimens were more than 2 cm, reaching a safe distance. The success rate of surgical resection was 100.0%.@*CONCLUSIONS@#In video-assisted thoracoscopic surgery for ground glass nodules of lung, the body surface theodolitic puncture localization method can be accurate, safe and simple.
ABSTRACT
Objective To study the changes of endotoxin in blood,Escherichia coli,lactobacillus,bifidobacterium,enterococci in feces of HCC patients.Methods Real-time fluorescence quantitative PCR was used to detect the difference between HCC group and normal group in the expression in bifidobacterium,lactobacillus,enterococcus and Escherichia coli,and the difference of endotoxin level.Results The expression levels of Escherichia coli and enterococcus in HCC group were significantly higher than those in normal control group (P＜ 0.05).Bifidobacterium and lactobacillus in primary liver cancer group significantly decreased compared with the normal group (P ＜ 0.05).The serum endotoxin level in HCC group was significantly higher than that in normal control group (P ＜ 0.05).Bifidobacterium and lactobacillus increased in HCC group.Enterococcus and escherichia coli decreased in HCC group.There was a significant positive correlation between endotoxin content and tumor number and tumor diameter in HCC patients.Conclusion Bifidobacterium,lactobacillus decreased,enterococcus and Escherichia coli increased in HCC patients.These changes along with the increase of endotoxin may relate to the development of HCC.
ABSTRACT
Objective The objective of this study was to investigate the effect of lycopene( LP) on the proliferation and apoptosis of human osteosarcoma MG-63 cells and to explore its mechanism. Methods MG-63 cells in the logarithmic growth phase were treated with LP(5,10 and 20μg/mL) or Cisplatin(40μg/mL) for 48 h. No LP or Ciplatin treatment was as the control group. The cell growth status was observed and the cell prolif-eration was measured by MTT colorimetric assay. Flow cytometry( FCM) was used to detect the cell cycle and ap-optosis of MG-63 cells and the apoptotic rate was calculated in this study. Reverse transcription-polymerase chain reaction(RT-PCR)was used to measure mRNA levels of Bax and bcl-2,and calculate the ratio of Bax/bcl-2 in MG-63 cells. Western blot also was used to examine the expression of caspase-3 protein in MG-63 cells. Results MG-63 cells were adherent and proliferated rapidly in the control group. The cells in LP treated groups showed that the cell proliferation was inhibited and the number of cells was significantly decreased,with retraction and detaching. The inhibitory rate of MG -63 cells in LP treated group were significantly increased when compared to the control group. LP significantly prolonged the cell cycle at the G0/G1 phase and shortened the cell cycle at the S and G2/M phase. LP also significantly increased the apoptosis rate,up-regulated the mR-NA level of Bax,down-regulated the mRNA level of bcl-2,increased the ratio of Bax/bcl-2 and the expres-sion of caspase-3 protein in MG-63 cells(P<0. 05 or P<0. 01). Conclusion LP may effectively inhibit the proliferation of MG-63 cells and promote the apoptosis. The mechanism may be related to the cell cycle and the regulation of apoptosis and gene expression.